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Related topics: Antibody Discovery, Protein Expression, Protein Concentration & Purification

9/2017

Vivaflow® 200: A critical sample preparation tool for concentrating hybridoma supernatants

This study focusses on the use of Vivaflow® 200 crossflow cassettes to concentrate up to 3 L clear murine hybridoma supernatant 10-fold prior to affinity chromatography. Consistent antibody recoveries in excess of 98 % at speeds of concentration around 20 – 25 mL/min are observed.

Author: Mike Brownleader, Ian Phillips, Andy Wright

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