don’t need superpowers but smart tools to obtain specific results in mycoplasma testing within 3 hours.

Highest Specificity Without Cultivating Living Mycoplasma for Weeks: With the Microsart® Mycoplasma Kits.

Why waste time waiting for reliable results? Viable, but non-culturable (VBNC) Mycoplasma go undetected when using the traditional method of mycoplasma culturing, risking false negative results. The Microsart® qPCR kits offer a method for early detection of mycoplasma contamination. Fast, highly specific and in line with international guidelines – both false positive and false negative results are practically ruled out. A safe and rapid solution. Because real heroes deserve only the best.

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Microsart® Mycoplasma Kits

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Mycoplasma Detection | Pharmaceutical Microbiology | Sartorius
  • Time-saving by avoiding lengthy 28 day cultivation protocols.
  • Ensures the highest level of qPCR specificity through use of TaqMan® probes.
  • No need to perform uncertain melting curve analysis.

Don’t worry about false positives – instead obtain reliable results within 3 hours:

Mycoplasma Detection | Pharmaceutical Microbiology | Sartorius

Ensure validated, quality test results in compliance with international standards:

  • Microsart® AMP & ATMP Mycoplasma Kits are validated according to EP 2.6.7 for sensitivity, specificity and robustness and fulfill international criteria of international authorities like USP and JP.
  • Developed in close cooperation with authorities.

Eliminate contamination risks by using the only kit with non-infectious mycoplasma cells:

  • Validation standards for required mycoplasma species are lyophilized to render them non-infectious. 
  • Detailed step-by-step guidance for matrix-specific validation provided by Sartorius qPCR experts.
Mycoplasma Detection | Pharmaceutical Microbiology | Sartorius

Let the master mix do the internal control and reduce tedious pipetting steps:

  • The DNA-sequence for internal control is included in the ready-to-use master mix.
  • Minimize false-negative results and the number of reactions.

Start testing right away using your existing equipment:

  • The kits can be used with any type of qPCR cycler able to detect the fluorescent dyes FAM® and ROX®.
  • Clear, color-coded reagents guide through the short and simple protocol.
  • Lyophilized reagents ensure consistent quality without the need for freezer storage.
Mycoplasma Detection | Pharmaceutical Microbiology | Sartorius
  • Compendial testing and release: For controlling raw materials, in-process testing, and lot release testing of finished products:
    Microsart® AMP Mycoplasma Kit
  • Compendial testing and release : For testing cell-based therapeutics (ATMPs) such as autologous chondrocyte transplants or CAR-T cells:
    Microsart® ATMP Mycoplasma Kit
  • In research & development: For detection of Mycoplasma DNA in cell culture supernatants:
    Microsart® RESEARCH Mycoplasma Kit

Choose the appropriate Microsart® Mycoplasma Kit based on your requirements:

Mycoplasma qPCR assays offer gold standard performance, design and configuration flexibility, affordability and manufacturing quality.

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Rapid real-time PCR Mycoplasma Detection Kit

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Rapid real-time PCR Mycoplasma Detection Kit for testing ATMPs

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Mycoplasma Detection Kit for qPCR

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Free Trial: Mycoplasma Detection Solutions

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Sartorius Services

BioOutsource Biosafety Testing Services

Sartorius Stedim BioOutsource offers sterility tests by Direct Inoculation and by Membrane Filtration to GMP regulations and in compliance with the European Pharmacopoeia (EP), Japanese Pharmacopoeia (JP) and the United States Pharmacopoeia (USP) standards.

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Secure and Cost Effective Mycoplasma Removal

Fully validated filter and membrane

Mycoplasma can be present in commercially available cell culture media and are able to penetrate 0.2 µm rated sterile filters.

That’s why Sartorius developed the Sartopore® 2 XLM filter with a 0.2 µm prefilter layer and the 0.1 µm final membrane for the successful retention of Mycoplasma. It is fully validated as a sterilizing-grade filter for the removal of Acholeplasma laidlawii with an LRV 7/cm² filter area.

Find out how to reduce the risk of mycoplasma contamination in your cell culture media | fermentation process.

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